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Author (up) Arista, S.; Ramirez, S.; De Grazia, S.; Giammanco, G. M.; Milici, M.; Colomba, C.; Ruggeri, F. M.; Martella, V. openurl 
  Title Detection of the norovirus variants GGII.4 hunter and GGIlb/hilversum in Italian children with gastroenteritis Type Journal Article
  Year 2006 Publication Journal of Medical Virology Abbreviated Journal  
  Volume 78 Issue 12 Pages 1656-1662  
  Corporate Author Thesis  
  Address Arista, S Univ Palermo, Dipartimento Igiene & Microbiol, Via Vespro 133, I-90127 Palermo, Italy Univ Palermo, Dipartimento Igiene & Microbiol, Via Vespro 133, I-90127 Palermo, Italy Univ Palermo, Dipartimento Igiene & Microbiol, I-90127 Palermo, Italy Univ Palermo, Ist Patol Infett & Virol, I-90127 Palermo, Italy Ist Super Sanita, Dipartimento Sicurezza Alimenti & Salute Pubbl An, I-00161 Rome, Italy Univ Bari, Dipartimento Sanita & Benessere Anim, Bari, Italy  
  Keywords norovirus genotyping enteritis children italy norwalk-like viruses round-structured viruses transcription-pcr assay molecular epidemiology recombinant noroviruses united-states viral gastroenteritis sequence alignment outbreaks caliciviruses  
  Abstract Noroviruses (NoVs) are important enteric pathogens of humans. Although they exhibit an impressive genetic diversity, few NoV strains appear to predominate worldwide. Limited epidemiological data are available on NoV gastroenteritis in Italy. In this study, we assessed the prevalence of human NoV in Italian children with gastroenteritis by using a reverse-transcription nested polymerase chain reaction (RT-PCR) assay specific for the RNA-dependent RNA polymerase (RdRp) on faecal samples collected throughout the 2004 surveillance activity in Palermo, Italy. NoVs were detected in 47% of the stool samples obtained from children < 5 years age, admitted to hospital with acute non-bacterial gastroenteritis. A selection of strains was further analyzed by partial sequence analysis of the RdRp gene. The strains were characterized as genogroup (GG) II and clustered into two distinct virus populations that resembled the emerging European GGIIb/Hilversum strains and the Australian Hunter GG11.4 strains. A temporal pattern of distribution of the two NoV strains was observed which was consistent with an independent circulation of two separate strains in the local population. Based on this 1-year study we concluded that NoVs were a diffuse cause of sporadic cases of acute childhood gastroenteritis and that strains of global epidemiological relevance were circulating in Palermo, Italy in 2004.  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0146-6615 ISBN Medium  
  Area Expedition Conference  
  Notes 102OP Times Cited:18 Cited References Count:38 Approved no  
  Call Number refbase @ user Serial 584  
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