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Author Moore, M.D.; Jaykus, L.-A. doi  openurl
  Title (up) A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability Type Research Support, U.S. Gov't, Non-P.H.S.
  Year 2017 Publication Analytical biochemistry Abbreviated Journal Anal Biochem  
  Volume 533 Issue Pages 56-59  
  Corporate Author Thesis  
  Address Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, 315 Schaub Hall, 400 Dan Allen Drive, Raleigh, NC 27695, USA  
  Keywords Amino Acid Sequence/genetics; Blood Group Antigens/immunology/*isolation & purification; Capsid Proteins/*blood; Gastroenteritis/diagnosis/*virology; Genotype; Humans; Norovirus/genetics/immunology/*isolation & purification/pathogenicity; Protein Binding; *Capsid functionality; *Disinfection; *Histo-blood group antigens; *Norovirus; *Receptor binding assay  
  Abstract Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid integrity. We present a streamlined version of a receptor-binding assay with minimal time-to-result while maintaining accuracy and high throughput. We validate assay performance for physical and chemical inactivation treatments of a norovirus GII.4 capsid. The assay produces a high positive/negative ratio (25.3 +/- 4.9) in <2.5 h and has a limit of detection of 0.1 mug/ml capsid. This method is a valuable additional tool for understanding human norovirus inactivation.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0003-2697 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28663001 Approved no  
  Call Number NCSU @ edshirle @ Serial 3611  
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