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Author Moore, M.D.; Escudero-Abarca, B.I.; Jaykus, L.-A. doi  openurl
  Title An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding Type Research Support, U.S. Gov't, Non-P.H.S.
  Year 2017 Publication Methods in molecular biology (Clifton, N.J.) Abbreviated Journal Methods Mol Biol  
  Volume (down) 1575 Issue Pages 291-302  
  Corporate Author Thesis  
  Address Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 315 Schaub Hall, 400 Dan Allen Drive, Raleigh, NC, 27695, USA  
  Keywords Aptamers, Nucleotide/genetics/*metabolism; Biotin/*chemistry; Capsid Proteins/*metabolism; DNA, Single-Stranded; Enzyme-Linked Immunosorbent Assay; Humans; Norovirus/classification/metabolism; SELEX Aptamer Technique/instrumentation; *Aptamer; *Aptamer binding; *Binding evaluation; *DNA-protein binding; *Enzyme-linked aptamer sorbent assay; *Plate assay  
  Abstract Nucleic acid aptamers are a class of alternative ligands increasingly growing in importance in the face of contemporary detection challenges. Aptamers offer multiple advantages over traditional ligands like antibodies; however, their ability to specifically bind target molecules must first be confirmed after their generation. Use of a plate-based enzyme-linked aptamer sorbent assay (ELASA) is a generally rapid way to screen and characterize aptamer binding to protein targets. ELASA involves directly plating a protein target onto a nonspecific (polystyrene) surface and assessing binding of functionalized (biotinylated) aptamers to those plated proteins using an enzyme conjugate that recognizes the aptamers. Here, we describe an ELASA that was designed and used to evaluate and compare binding of ssDNA aptamers against the capsids of different strains of human norovirus.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1064-3745 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28255888 Approved no  
  Call Number NCSU @ edshirle @ Serial 3610  
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