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Author Marti, E.; Ferrary-Americo, M.; Barardi, C.R.M. doi  openurl
  Title Detection of Potential Infectious Enteric Viruses in Fresh Produce by (RT)-qPCR Preceded by Nuclease Treatment Type Research Support, Non-U.S. Gov't
  Year (down) 2017 Publication Food and environmental virology Abbreviated Journal Food Environ Virol  
  Volume 9 Issue 4 Pages 444-452  
  Corporate Author Thesis  
  Address Laboratorio de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciencias Biologicas, Universidade Federal de Santa Catarina, Florianopolis, SC, CEP: 88040-970, Brazil  
  Keywords Adenoviruses, Human/chemistry/*genetics/isolation & purification; Biocatalysis; Deoxyribonucleases/chemistry; Food Contamination/analysis; Hepatitis A virus/chemistry/genetics/isolation & purification; Lettuce/virology; Norovirus/*genetics/isolation & purification; Real-Time Polymerase Chain Reaction; *(RT)-qPCR; *DNase; *Enteric viruses; *Fresh produce; *RNase  
  Abstract Foodborne illnesses associated with contaminated fresh produce are a common public health problem and there is an upward trend of outbreaks caused by enteric viruses, especially human noroviruses (HNoVs) and hepatitis A virus (HAV). This study aimed to assess the use of DNase and RNase coupled to qPCR and RT-qPCR, respectively, to detect intact particles of human adenoviruses (HAdVs), HNoV GI and GII and HAV in fresh produce. Different concentrations of DNase and RNase were tested to optimize the degradation of free DNA and RNA from inactivated HAdV and murine norovirus (MNV), respectively. Results indicated that 10 microg/ml of RNase was able to degrade more than 4 log10 (99.99%) of free RNA, and 1 U of DNase degraded the range of 0.84-2.5 log10 of free DNA depending on the fresh produce analysed. The treatment with nucleases coupled to (RT)-qPCR was applied to detect potential infectious virus in organic lettuce, green onions and strawberries collected in different seasons. As a result, no intact particles of HNoV GI and GII were detected in the 36 samples analysed, HAdV was found in one sample and HAV was present in 33.3% of the samples, without any reasonable distribution pattern among seasons. In conclusion, RT-qPCR preceded by RNase treatment of eluted samples from fresh produce is a good alternative to detect undamaged RNA viruses and therefore, potential infectious viruses. Moreover, this study provides data about the prevalence of enteric viruses in organic fresh produce from Brazil.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1867-0334 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28452009 Approved no  
  Call Number NCSU @ edshirle @ Serial 3600  
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