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Author Ma, L.; Su, L.; Liu, H.; Zhao, F.; Zhou, D.; Duan, D. doi  openurl
  Title Norovirus contamination and the glycosphingolipid biosynthesis pathway in Pacific oyster: A transcriptomics study Type Journal Article
  Year 2017 Publication Fish & shellfish immunology Abbreviated Journal Fish Shellfish Immunol  
  Volume 66 Issue Pages 26-34  
  Corporate Author Thesis  
  Address Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong, 266071, China. Electronic address: dlduan@qdio.ac.cn  
  Keywords Animals; Biosynthetic Pathways; Crassostrea/*metabolism/*virology; Glycosphingolipids/*metabolism; Norovirus/*physiology; Sequence Analysis, RNA; *Transcriptome; *Up-Regulation; Crassostrea gigas; Glycosphingolipid biosynthesis pathway; Norovirus; Transcriptome  
  Abstract Noroviruses are the primary pathogens associated with shellfish-borne gastroenteritis outbreaks. These viruses remain stable in oysters, suggesting an active mechanism for virus concentration. In this study, a deep RNA sequencing technique was used to analyze the transcriptome profiles of Pacific oysters at different time points after inoculation with norovirus (GII.4). We obtained a maximum of 65, 294, 698 clean sample reads. When aligned to the reference genome, the average mapping ratio of clean data was approximately 65%. In the samples harvested at 12, 24, and 48 h after contamination, 2,223, 2,990, and 2020 genes, respectively, were differentially expressed in contaminated and non-contaminated oyster digestive tissues, including 500, 1748, and 1039 up-regulated and 1723, 1242, and 981 down-regulated genes, respectively. In particular, FUT2 and B3GNT4, genes encoding the signaling components of glycosphingolipid biosynthesis, were significantly up-regulated in contaminated samples. In addition, we found up-regulation of some immune- and disease-related genes in the MHC I pathway (PA28, HSP 70, HSP90, CANX, BRp57, and CALR) and MHC II pathway (GILT, CTSBLS, RFX, and NFY), although NoVs did not cause diseases in the oysters. We detected two types of HBGA-like molecules with positive-to-negative ratios similar to type A and H1 HBGA-like molecules in digestive tissues that were significantly higher in norovirus-contaminated than in non-contaminated oysters. Thus, our transcriptome data analysis indicated that a human pathogen (GII.4 Norovirus) was likely concentrated in the digestive tissues of oysters via HBGA-like molecules that were synthesized by the glycosphingolipid biosynthesis pathway. The identified differentially expressed genes also provide potential candidates for functional analysis to identify genes involved in the accumulation of noroviruses in oysters.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1050-4648 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28457919 Approved no  
  Call Number NCSU @ edshirle @ Serial 3598  
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