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Author (up) Kim, D.; Lee, H.-M.; Oh, K.-S.; Ki, A.Y.; Protzman, R.A.; Kim, D.; Choi, J.-S.; Kim, M.J.; Kim, S.H.; Vaidya, B.; Lee, S.J.; Kwon, J. doi  openurl
  Title Exploration of the metal coordination region of concanavalin A for its interaction with human norovirus Type Research Support, Non-U.S. Gov't
  Year 2017 Publication Biomaterials Abbreviated Journal Biomaterials  
  Volume 128 Issue Pages 33-43  
  Corporate Author Thesis  
  Address Biological Disaster Analysis Group, Korea Basic Science Institute, Gwahak-ro, Yuseong-gu, Daejeon 34133, Republic of Korea. Electronic address: joseph@kbsi.re.kr  
  Keywords Acrylic Resins/chemistry; Amino Acid Sequence; Concanavalin A/*pharmacology; Deuterium Exchange Measurement; Food Microbiology; Humans; Metals/*pharmacology; Microspheres; Norovirus/*drug effects; Plant Lectins/chemistry; Soybean Proteins/chemistry; *Concanavalin A; *Human norovirus (GI and GII); *Lectin; *Metal coordination; *Polyacrylamide bead  
  Abstract Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are needed to control foodborne disease outbreaks, but reliable techniques that are fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments are not yet available. We explore the interactions between HuNoV and concanavalin A (Con A), which could facilitate the development of a sensitive detection tool for HuNoV. Biophysical studies including hydrogen/deuterium exchange (HDX) mass spectrometry and surface plasmon resonance (SPR) revealed that when the metal coordinated region of Con A, which spans Asp16 to His24, is converted to nine alanine residues (mCon A(MCR)), the affinity for HuNoV (GII.4) diminishes, demonstrating that this Ca(2+) and Mn(2+) coordinated region is responsible for the observed virus-protein interaction. The mutated carbohydrate binding region of Con A (mCon A(CBR)) does not affect binding affinity significantly, indicating that MCR of Con A is a major region of interaction to HuNoV (GII.4). The results further contribute to the development of a HuNoV concentration tool, Con A-immobilized polyacrylate beads (Con A-PAB), for rapid detection of genotypes from genogroups I and II (GI and GII). This method offers many advantages over currently available methods, including a short concentration time. HuNov (GI and GII) can be detected in just 15 min with 90% recovery through Con A-PAB application. In addition, this method can be used over a wide range of pH values (pH 3.0 – 10.0). Overall, this rapid and sensitive detection of HuNoV (GI and GII) will aid in the prevention of virus transmission pathways, and the method developed here may have applicability for other foodborne viral infections.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0142-9612 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28288347 Approved no  
  Call Number NCSU @ edshirle @ Serial 3580  
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