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Author Fan, W.-L.; Wang, Z.-W.; Qin, Y.; Sun, C.; Liu, Z.-M.; Jiang, Y.-P.; Qiao, X.-Y.; Tang, L.-J.; Li, Y.-J.; Xu, Y.-G. doi  openurl
  Title Use of dual priming oligonucleotide system-based multiplex RT-PCR combined with high performance liquid chromatography assay for simultaneous detection of five enteric viruses associated with acute enteritis Type Research Support, Non-U.S. Gov't
  Year (down) 2017 Publication Journal of virological methods Abbreviated Journal J Virol Methods  
  Volume 243 Issue Pages 80-82  
  Corporate Author Thesis  
  Address College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China. Electronic address:  
  Keywords Chromatography, High Pressure Liquid/*methods; Enteritis/*diagnosis; Humans; Molecular Diagnostic Techniques/*methods; Multiplex Polymerase Chain Reaction/*methods; Reverse Transcriptase Polymerase Chain Reaction/*methods; Sensitivity and Specificity; Virus Diseases/*diagnosis; *Accurate diagnosis; *DPO system-based mRT-PCR-HPLC assay; *Five enteric viruses associated with acute enteritis  
  Abstract In this study, a specific and sensitive method for simultaneous detection of human astrovirus, human rotavirus, norovirus, sapovirus and enteric adenovirus associated with acute enteritis was developed, based on the specific dual priming oligonucleotide (DPO) system and the sensitive high-performance liquid chromatography (HPLC) analysis. The DPO system-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) combined with HPLC assay was more sensitive than agarose gel electrophoresis analysis and real-time SYBR Green PCR assay, and showed a specificity of 100% and sensitivity of 96%-100%. The high sensitivity and specificity of the assay indicates its great potential to be a useful tool for the accurate diagnosis of enteric virus infections.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0166-0934 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28185831 Approved no  
  Call Number NCSU @ edshirle @ Serial 3543  
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