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Author Cho, S.; Park, T.S.; Reynolds, K.A.; Yoon, J.-Y. doi  openurl
  Title Multi-Normalization and Interpolation Protocol to Improve Norovirus Immunoagglutination Assay from Paper Microfluidics with Smartphone Detection Type Journal Article
  Year (down) 2017 Publication SLAS technology Abbreviated Journal SLAS Technol  
  Volume 22 Issue 6 Pages 609-615  
  Corporate Author Thesis  
  Address 4 Department of Biomedical Engineering, The University of Arizona, Tucson, AZ, USA  
  Keywords biosensor; immunoassay; smartphone; water safety; microPAD  
  Abstract Norovirus (NoV) is one of the leading causes of acute gastroenteritis, affecting 685 million people per year around the world. The best preventive measure is to screen water for possible NoV contamination, not from infected humans, preferably using rapid and field-deployable diagnostic methods. While enzyme immunoassays (EIAs) can be used for such detection, the low infectious dose as well as the generally inferior sensitivity and low titer of available NoV antibodies render critical challenges in using EIAs toward NoV detection. In this work, we demonstrated smartphone-based Mie scatter detection of NoV with immunoagglutinated latex particles on paper microfluidic chips. Using only three different concentrations of anti-NoV-conjugated particles, we were able to construct a single standard curve that covered seven orders of magnitude of NoV antigen concentrations. Multiple normalization steps and interpolation procedures were developed to estimate the optimum amount of antibody-conjugated particles that matched to the target NoV concentration. A very low detection limit of 10 pg/mL was achieved without using any concentration or enrichment steps. This method can also be adapted for detection of any other virus pathogens whose antibodies possess low sensitivity and low antibody titer.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2472-6303 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28813186 Approved no  
  Call Number NCSU @ edshirle @ Serial 3521  
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