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Author (up) Almand, E.A.; Moore, M.D.; Outlaw, J.; Jaykus, L.-A. doi  openurl
  Title Human norovirus binding to select bacteria representative of the human gut microbiota Type Journal Article
  Year 2017 Publication PloS one Abbreviated Journal PLoS One  
  Volume 12 Issue 3 Pages e0173124  
  Corporate Author Thesis  
  Address Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC, United States of America  
  Keywords Bacillus/isolation & purification/metabolism; Citrobacter/isolation & purification/metabolism; Culture Media/chemistry; Enterobacter cloacae/isolation & purification/metabolism; Enterococcus faecium/isolation & purification/metabolism; Feces/microbiology; Fimbriae, Bacterial/chemistry/*metabolism/ultrastructure; Gastrointestinal Microbiome/*physiology; Hafnia alvei/isolation & purification/metabolism; Humans; Klebsiella/isolation & purification/metabolism; *Microbial Interactions; Norovirus/*metabolism/ultrastructure; Staphylococcus aureus/isolation & purification/metabolism  
  Abstract Recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. The purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. The bacteria screened included naturally occurring strains isolated from human stool (Klebsiella spp., Citrobacter spp., Bacillus spp., Enterococcus faecium and Hafnia alvei) and select reference strains (Staphylococcus aureus and Enterobacter cloacae). Binding in PBS was evaluated to three human norovirus strains (GII.4 New Orleans 2009 and Sydney 2012, GI.6) and two surrogate viruses (Tulane virus and Turnip Crinkle Virus (TCV)) using a suspension assay format linked to RT-qPCR for quantification. The impact of different overnight culture media prior to washing on binding efficiency in PBS was also evaluated, and binding was visualized using transmission electron microscopy. All bacteria tested bound the representative human norovirus strains with high efficiency (<1 log10 of input virus remained unbound or <10% unbound and >90% binding efficiency) (p>0.05); there was selective binding for Tulane virus and no binding observed for TCV. Binding efficiency was highest when bacteria were cultured in minimal media (<1 log10 of input virus remained unbound, so >90% bound), but notably decreased when cultured in enriched media (1-3 log10 unbound or 0.01 -<90% bound)) (p<0.05). The norovirus-bacteria binding occurred around the outer cell surfaces and pili structures, without apparent localization. The findings reported here further elucidate and inform the dynamics between human noroviruses and enteric bacteria with implications for norovirus pathogenesis.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1932-6203 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28257478 Approved no  
  Call Number NCSU @ edshirle @ Serial 3497  
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