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Author (up) Alberer, M.; Schlenker, N.; Bauer, M.; Helfrich, K.; Mengele, C.; Loscher, T.; Nothdurft, H.D.; Bretzel, G.; Beissner, M. doi  openurl
  Title Detection of Gastrointestinal Pathogens from Stool Samples on Hemoccult Cards by Multiplex PCR Type Journal Article
  Year 2017 Publication The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale Abbreviated Journal Can J Infect Dis Med Microbiol  
  Volume 2017 Issue Pages 3472537  
  Corporate Author Thesis  
  Address Department of Infectious Diseases and Tropical Medicine, Medical Centre, Ludwig-Maximilians-University (LMU), Munich, Germany  
  Keywords  
  Abstract Purpose. Up to 30% of international travelers are affected by travelers' diarrhea (TD). Reliable data on the etiology of TD is lacking. Sufficient laboratory capacity at travel destinations is often unavailable and transporting conventional stool samples to the home country is inconvenient. We evaluated the use of Hemoccult cards for stool sampling combined with a multiplex PCR for the detection of model viral, bacterial, and protozoal TD pathogens. Methods. Following the creation of serial dilutions for each model pathogen, last positive dilution steps (LPDs) and thereof calculated last positive sample concentrations (LPCs) were compared between conventional stool samples and card samples. Furthermore, card samples were tested after a prolonged time interval simulating storage during a travel duration of up to 6 weeks. Results. The LPDs/LPCs were comparable to testing of conventional stool samples. After storage on Hemoccult cards, the recovery rate was 97.6% for C. jejuni, 100% for E. histolytica, 97.6% for norovirus GI, and 100% for GII. Detection of expected pathogens was possible at weekly intervals up to 42 days. Conclusion. Stool samples on Hemoccult cards stored at room temperature can be used in combination with a multiplex PCR as a reliable tool for testing of TD pathogens.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1712-9532 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28408937 Approved no  
  Call Number NCSU @ edshirle @ Serial 3495  
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