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Author Takemura, K.; Adegoke, O.; Takahashi, N.; Kato, T.; Li, T.-C.; Kitamoto, N.; Tanaka, T.; Suzuki, T.; Park, E.Y. doi  openurl
  Title (down) Versatility of a localized surface plasmon resonance-based gold nanoparticle-alloyed quantum dot nanobiosensor for immunofluorescence detection of viruses Type Journal Article
  Year 2017 Publication Biosensors & bioelectronics Abbreviated Journal Biosens Bioelectron  
  Volume 89 Issue Pt 2 Pages 998-1005  
  Corporate Author Thesis  
  Address Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp  
  Keywords *Biosensing Techniques; Cysteine/chemistry; Gold/chemistry; Hemagglutinin Glycoproteins, Influenza Virus/*isolation & purification; Humans; Influenza A Virus, H1N1 Subtype/chemistry/*isolation & purification; Influenza A Virus, H3N2 Subtype/chemistry/*isolation & purification; Influenza, Human/*diagnosis/virology; Limit of Detection; Metal Nanoparticles/chemistry; Quantum Dots/chemistry; Surface Plasmon Resonance; *Alloyed quantum dot; *Gold nanoparticle; *Immunofluorescence detection; *Influenza virus; *Localized surface plasmon resonance; *Norovirus  
  Abstract Flu infection, caused by the influenza virus, constitutes a serious threat to human lives worldwide. A rapid, sensitive and specific diagnosis is urgently needed for point-of-care treatment and to control the rapid spread of this disease. In this study, an ultrasensitive, rapid and specific localized surface plasmon resonance (LSPR)-induced immunofluorescence nanobiosensor has been developed for the influenza virus based on a gold nanoparticle (AuNP)-induced quantum dot (QD) fluorescence signal. Alloyed quaternary CdSeTeS QDs were synthesized via the hot-injection organometallic route and were subsequently capped with l-cysteine via a ligand exchange reaction. AuNPs were synthesized in HEPES buffer and thiolated with l-cysteine. The concept of the biosensor involves the conjugation of anti-neuraminidase (NA) antibody (anti-NA Ab) to thiolated AuNPs and the conjugation of anti-hemagglutinin (HA) antibody (anti-HA Ab) to alloyed quaternary l-cysteine-capped CdSeTeS QDs. Interaction of the antigens displaying on the surface of the influenza virus target with anti-NA Ab-conjugated AuNPs and anti-HA Ab-conjugated QDs induces an LSPR signal from adjacent AuNPs to trigger fluorescence-enhancement changes in the QDs in proportion to the concentration of the target virus. The detection limit for influenza H1N1 virus was 0.03pg/mL in deionized water and 0.4pg/mL in human serum; while, for the clinically isolated H3N2, the detection limit was 10PFU/mL. The detection of influenza virus H1N1 was accomplished with high sensitivity. The versatility of the biosensor was demonstrated for the detection of clinically isolated influenza virus H3N2 and norovirus-like particles (NoV-LPs).  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0956-5663 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:27825520 Approved no  
  Call Number NCSU @ edshirle @ Serial 3475  
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