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Author Hwang, H.J.; Ryu, M.Y.; Park, C.Y.; Ahn, J.; Park, H.G.; Choi, C.; Ha, S.-D.; Park, T.J.; Park, J.P. doi  openurl
  Title High sensitive and selective electrochemical biosensor: Label-free detection of human norovirus using affinity peptide as molecular binder Type Journal Article
  Year (down) 2017 Publication Biosensors & bioelectronics Abbreviated Journal Biosens Bioelectron  
  Volume 87 Issue Pages 164-170  
  Corporate Author Thesis  
  Address Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, Republic of Korea. Electronic address: jppark@dhu.ac.kr  
  Keywords Amino Acid Sequence; Animals; Biosensing Techniques/instrumentation/*methods; Caliciviridae Infections/blood/diagnosis/*virology; Cattle; Cysteine/chemistry; Electrochemical Techniques/instrumentation/methods; Equipment Design; Gold/*chemistry; Humans; Limit of Detection; Models, Molecular; Norovirus/*isolation & purification; Peptide Library; Peptides/*chemistry; Point-of-Care Testing; Affinity peptide; Biosensor; Human norovirus; Limit of detection; Molecular binder; Sensitivity  
  Abstract Norovirus is known as the major cause of highly infection for gastrointestinal tracts. In this study, robust and highly sensitive biosensors for detecting human norovirus by employing a recognition affinity peptide-based electrochemical platform were described. A series of amino acid-substituted and cysteine-incorporated recognition peptides isolated from evolutionary phage display technique was chemically synthesized and immobilized to a gold sensor layer, the detection performance of the gold-immobilized synthetic peptide-based sensor system was assessed using QCM, CV and EIS. Using EIS, the limit of detection with Noro-1 as a molecular binder was found to be 99.8nM for recombinant noroviral capsid proteins (rP2) and 7.8copies/mL for human norovirus, thereby demonstrating a high degree of sensitivity for their corresponding targets. These results suggest that a biosensor which consists of affinity peptides as a molecular binder and miniaturized microdevices as diagnostic tool could be served as a new type of biosensing platform for point-of-care testing.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0956-5663 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:27551996 Approved no  
  Call Number NCSU @ edshirle @ Serial 3400  
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