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Author (up) Ashiba, H.; Sugiyama, Y.; Wang, X.; Shirato, H.; Higo-Moriguchi, K.; Taniguchi, K.; Ohki, Y.; Fujimaki, M. doi  openurl
  Title Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels Type Journal Article
  Year 2017 Publication Biosensors & bioelectronics Abbreviated Journal Biosens Bioelectron  
  Volume 93 Issue Pages 260-266  
  Corporate Author Thesis  
  Address Electronics and Photonics Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan  
  Keywords *Biosensing Techniques; Caliciviridae Infections/diagnosis/*virology; Fluorescent Dyes/chemistry; Humans; Norovirus/*isolation & purification/pathogenicity; Quantum Dots; Signal-To-Noise Ratio; Surface Plasmon Resonance; Norovirus detection; Quantum dot; Sensitivity enhancement; Sensor design; Surface plasmon resonance; V-trench biosensor  
  Abstract A highly sensitive biosensor to detect norovirus in environment is desired to prevent the spread of infection. In this study, we investigated a design of surface plasmon resonance (SPR)-assisted fluoroimmunosensor to increase its sensitivity and performed detection of norovirus virus-like particles (VLPs). A quantum dot fluorescent dye was employed because of its large Stokes shift. The sensor design was optimized for the CdSe-ZnS-based quantum dots. The optimal design was applied to a simple SPR-assisted fluoroimmunosensor that uses a sensor chip equipped with a V-shaped trench. Excitation efficiency of the quantum dots, degree of electric field enhancement by SPR, and intensity of autofluorescence of a substrate of the sensor chip were theoretically and experimentally evaluated to maximize the signal-to-noise ratio. As the result, an excitation wavelength of 390nm was selected to excite SPR on an Al film of the sensor chip. The sandwich assay of norovirus VLPs was performed using the designed sensor. Minimum detectable concentration of 0.01ng/mL, which corresponds to 100 virus-like particles included in the detection region of the V-trench, was demonstrated.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0956-5663 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:27597126 Approved no  
  Call Number NCSU @ edshirle @ Serial 3361  
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