||AIMS: Human norovirus (HuNoV) is estimated to cause 19-21 million illnesses each year in the US. A major limitation in HuNoV research is the lack of an in vitro culture system; therefore, surrogate viruses including murine norovirus (MNV) and feline calicivirus (FCV) are used to study HuNoV. Here, we aim to establish the physiochemical properties of Tulane virus (TV)-a newer HuNoV surrogate. METHODS AND RESULTS: For thermal inactivation, TV was exposed to 37 degrees C for 2 h, and 56, 63 and 72 degrees C for 30 min. For ethanol tolerance, TV was treated with 60, 70 and 90% ethanol at room temperature (RT) for 5 min. Tulane virus pH stability at pH 2, 3, 7, 9 and 10 was performed at RT for 90 min. At 37 degrees C, there was no significant reduction in TV after 2 h. However, at 56, 63 and 72 degrees C, D-values of 4.03, 1.18, and 0.24 min, were calculated respectively. The D-values obtained for TV ethanol tolerance were 1.46, 1.93, and 0.35 min at 60, 70 and 90% respectively. Less than 1 log10 plaque forming units (PFU) reduction was observed for TV at all pH levels except pH 10 where about a 2-log10 PFU reduction was observed. Tulane virus was also tolerant to chlorine disinfection on a solid surface with D-values of 15.82 and 5.42 min at 200 and 1000 ppm respectively. CONCLUSIONS: Tulane virus is likely a suitable surrogate to study HuNoV thermal stability as well as ethanol tolerance below 90%. Tulane virus also is a promising surrogate to study HuNoV pH stability and chlorine tolerance. SIGNIFICANCE AND IMPACT OF THE STUDY: Based on current work, in vitro studies demonstrate that TV is an overall more conservative and suitable surrogate for the study of HuNoV physicochemical properties.