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Author (up) Ao, Y.-yun; Yu, J.-mei; Li, L.-li; Jin, M.; Duan, Z.-jun doi  openurl
  Title Detection of human norovirus GIV.1 in China: a case report Type Research Support, Non-U.S. Gov't
  Year 2014 Publication Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Abbreviated Journal J Clin Virol  
  Volume 61 Issue 2 Pages 298-301  
  Corporate Author Thesis  
  Address National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China. Electronic address:  
  Keywords Complete genome; Genogroup IV noroviruses; Phylogenetic analysis; Real-time PCR; Seminested PCR  
  Abstract Noroviruses (NoVs) are a common cause of acute gastroenteritis (AGE) around the world; however, reports of genogroup IV (GIV) NoVs are rare. Here we report a human GIV genotype 1 (GIV.1) NoV strain (named CHNNGIV2011) identified by 454 high-throughput sequencing from stool samples of children with diarrhea. This is the first documented human GIV.1 NoVs infection in China. The complete genome of the virus is 7525 nucleotides in length. Sequencing and phylogenetic analyses showed that CHNNGIV2011 shared high sequence similarity to other GIV.1 NoVs from all over the world, especially to the recently reported NoV GIV.1 strain Lake Macquarie genome (99.0%). By seminested PCR and real-time PCR, a total of 2 out of 466 samples were positive for GIV.1 CHNNGIV2011 in Lulong County, Hebei Province, China, which supported a low prevalence of GIV.1 NoVs. The positive samples contained 7.2x10(7) and 2.6x10(8)copies/g in feces. In addition, one positive sample was found coinfection with strains NoV GII and salivirus. These findings suggest more study is needed to address the worldwide prevalence and role of GIV NoVs in AGE.  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1386-6532 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:25151627 Approved no  
  Call Number NCSU @ edshirle @ Serial 2971  
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