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Author (up) Arcangeli, G.; Terregino, C.; De Benedictis, P.; Zecchin, B.; Manfrin, A.; Rossetti, E.; Magnabosco, C.; Mancin, M.; Brutti, A. url  openurl
  Title Effect of high hydrostatic pressure on murine norovirus in Manila clams Type Journal Article
  Year 2012 Publication Letters in Applied Microbiology Abbreviated Journal  
  Volume 54 Issue 4 Pages 325-9  
  Corporate Author Thesis  
  Address Istituto Zooprofilattico Sperimentale delle Venezie, V.le dell'Universita, Legnaro, PD, Italy. garcangeli@izsvenezie.it  
  Keywords  
  Abstract AIMS: Eating raw or insufficiently cooked bivalve molluscs contaminated with human noroviruses (NVs) can result in acute cases of gastroenteritis in humans. Manila clams (Ruditapes philippinarum) are particularly prone to exposure to NVs due to the brackish environment in which they are farmed which is known to be susceptible to human faecal contamination. High hydrostatic pressure processing (HHP) is a food treatment technique that has been shown to inactivate NV. METHODS AND RESULTS: In this study we investigated the ability of HHP to inactivate murine norovirus (MNV-1), a recognised surrogate for NV, in experimentally contaminated manila clams. Pools of contaminated live clams were subjected to hydrostatic pressure ranging from 300 to 500 MPa for different time intervals of between one and 10 min. The trial was repeated three times, at monthly intervals. CONCLUSIONS: Virus vitality post-treatment was assessed and the data obtained indicates that the use of high hydrostatic pressures of at least 500 MPa for 1 min was effective in inactivating MNV-1. SIGNIFICANCE AND IMPACT OF THE STUDY: HHP results to be an effective technique that could be applied to industrial process to obtain safe Manila clams ready to eat.  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1472-765X (Electronic) 0266-8254 (Linking) ISBN Medium  
  Area Expedition Conference  
  Notes Arcangeli, G Terregino, C De Benedictis, P Zecchin, B Manfrin, A Rossetti, E Magnabosco, C Mancin, M Brutti, A England Lett Appl Microbiol. 2012 Apr;54(4):325-9. doi: 10.1111/j.1472-765X.2012.03211.x. Epub 2012 Feb 15. Approved no  
  Call Number refbase @ user Serial 2292  
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